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Test Instructions

Simple, step-by-step instructions for your CareFree Kits test.

Select the test you have below. Please read the instructions for your test before you begin.

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Vitamin D Test Kit

Before you start

You will need:

  • Your Vitamin D test kit
  • The finger-prick lancet and blood collection equipment provided
  • The buffer provided
  • A timer or your phone

Before starting:

  • Read all instructions before beginning.
  • Room temperature matters: let the kit warm up in the room you will test in until it reaches room temperature (10–30°C) before you start.
  • Check the expiry date and ensure the packaging is undamaged.
  • Place the test cassette on a clean, flat surface.

Step 1 — Collect your blood sample

  • Wash your hands with warm water and soap, then dry them thoroughly.
  • Remove the protective cap from the single-use finger-prick lancet.
  • Press the lancet firmly against the side of a clean fingertip to prick your finger.
  • Hold your hand pointing down and gently massage from your palm towards the fingertip until a rounded drop of blood forms. Do not squeeze the puncture site.
  • Use the blood collection equipment in the kit to collect the blood drop.
  • Use the blood immediately, then safely dispose of the used lancet. Do not reuse it.
  • Take care when handling blood and avoid getting blood on your skin, eyes or other surfaces.

Step 2 — Add the blood

  • Add 2 drops of blood (approximately 10 µL) to the sample well marked S on the test cassette.

Step 3 — Add the buffer

  • Once the blood has soaked into the test, add 3 drops of the supplied buffer (approximately 90 µL) to the same sample well marked S.

Step 4 — Wait

  • Set a timer for 15–20 minutes.
  • Read your result within this time.
  • Do not interpret the test after 20 minutes.

Result timing

Read your result at 15–20 minutes.
Do not interpret the test after 20 minutes.

Understanding Your Result

1 — Check the control line (C)

A blue line must appear at C for the test to be valid. If no C line appears, the test is invalid. Repeat using a new test kit.

2 — Compare the test line (T)

Use the Vitamin D colour reference card below. Compare the colour of the T line with the reference bands. The lighter the T line, the lower the estimated Vitamin D level.

These are screening estimates, not a laboratory measurement or medical diagnosis.

CareFree Kits

Vitamin D — Colour Reference Card

Compare the colour of the T line with the colour bands.

Deficient

Below 10 ng/mL

Lighter than the 10 ng/mL reference

Insufficient

10 to below 30 ng/mL

Between the 10 and 30 ng/mL references

Normal range

30 ng/mL or above

Equal to or darker than the 30 ng/mL reference

The lighter the T line, the lower the estimated Vitamin D level.

Important information

  • Single use only. Do not reuse.
  • Store at 4–30°C. Do not freeze.
  • Test in a room between 10–30°C. If your room is colder or hotter, wait until it reaches this range before you start.
  • If you are concerned about your result, contact a healthcare professional.

Important information

Important information for all tests.

  • Check the expiry date. Never use a test that is expired, damaged or already open.
  • Only use the swab and items supplied with your kit.
  • Use a timer and read your result at the exact time stated. Never read it late.
  • No C line means the test is invalid. Repeat it with a new kit.
  • A negative result does not always rule out infection. Testing too soon, or collecting too little sample, can affect it.
  • A positive or reactive result should always be followed up with a healthcare professional.
  • Still have symptoms or worried about a recent exposure? Speak to a healthcare professional, even if your result is negative.
  • Not sure what your result shows? Do not guess. Repeat the test with a new kit or speak to a healthcare professional.
  • Keep test kits away from children and pets, and dispose of used items safely.
  • CareFree Kits provides testing products and general information. Our tests do not replace professional medical advice or diagnosis.

Need more help?

If you're unsure about your result or what to do next, speak with a qualified healthcare professional.

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