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Vitamin D Test Kit

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Vitamin D Test Kit

Before you start

You will need:

  • Your Vitamin D test kit
  • The finger-prick lancet and blood collection equipment provided
  • The buffer provided
  • A timer or your phone

Before starting:

  • Read all instructions before beginning.
  • Room temperature matters: let the kit warm up in the room you will test in until it reaches room temperature (10–30°C) before you start.
  • Check the expiry date and ensure the packaging is undamaged.
  • Place the test cassette on a clean, flat surface.

Step 1 — Collect your blood sample

  • Wash your hands with warm water and soap, then dry them thoroughly.
  • Remove the protective cap from the single-use finger-prick lancet.
  • Press the lancet firmly against the side of a clean fingertip to prick your finger.
  • Hold your hand pointing down and gently massage from your palm towards the fingertip until a rounded drop of blood forms. Do not squeeze the puncture site.
  • Use the blood collection equipment in the kit to collect the blood drop.
  • Use the blood immediately, then safely dispose of the used lancet. Do not reuse it.
  • Take care when handling blood and avoid getting blood on your skin, eyes or other surfaces.

Step 2 — Add the blood

  • Add 2 drops of blood (approximately 10 µL) to the sample well marked S on the test cassette.

Step 3 — Add the buffer

  • Once the blood has soaked into the test, add 3 drops of the supplied buffer (approximately 90 µL) to the same sample well marked S.

Step 4 — Wait

  • Set a timer for 15–20 minutes.
  • Read your result within this time.
  • Do not interpret the test after 20 minutes.

Result timing

Read your result at 15–20 minutes.
Do not interpret the test after 20 minutes.

Understanding Your Result

1 — Check the control line (C)

A blue line must appear at C for the test to be valid. If no C line appears, the test is invalid. Repeat using a new test kit.

2 — Compare the test line (T)

Use the Vitamin D colour reference card below. Compare the colour of the T line with the reference bands. The lighter the T line, the lower the estimated Vitamin D level.

These are screening estimates, not a laboratory measurement or medical diagnosis.

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Vitamin D — Colour Reference Card

Compare the colour of the T line with the colour bands.

Deficient

Below 10 ng/mL

Lighter than the 10 ng/mL reference

Insufficient

10 to below 30 ng/mL

Between the 10 and 30 ng/mL references

Normal range

30 ng/mL or above

Equal to or darker than the 30 ng/mL reference

The lighter the T line, the lower the estimated Vitamin D level.

Important information

  • Single use only. Do not reuse.
  • Store at 4–30°C. Do not freeze.
  • Test in a room between 10–30°C. If your room is colder or hotter, wait until it reaches this range before you start.
  • If you are concerned about your result, contact a healthcare professional.

My Result Summary

Create a simple PDF record of the result you read, to keep or show a healthcare professional.

This kit is read against the colour card in its instructions, so a result summary isn't available. If you're concerned about your result, speak to a healthcare professional.

What to do next

Check the control line first. If it is absent, the test is invalid and you should repeat with a new kit. Compare a valid test line using your kit reference card. If the estimate is low, unclear or inconsistent with how you feel, speak to a healthcare professional; do not choose treatment from this screening result alone.

This test does not measure other vitamins, diagnose a condition or replace a laboratory Vitamin D blood test. Screen colours are not a validated substitute for the original reference card. Discuss unexpected results or symptoms with a healthcare professional.

Call NHS 111Email CareFree Kits

In an emergency, call 999.

Find Healthcare Support Near You

If you have concerns about your vitamin D result, contact your GP to discuss whether assessment or testing is appropriate.

Find a GP
NHS 111 online Call NHS 111 Emergency 999

NHS 111 online is for people aged 5 and over. Call 111 for a child under 5. In an emergency, call 999.

CareFree Kits is a private service and is not affiliated with or operated by the NHS.

Important information

Important information for all tests.

  • Check the expiry date. Never use a test that is expired, damaged or already open.
  • Only use the swab and items supplied with your kit.
  • Use a timer and read your result at the exact time stated. Never read it late.
  • No C line means the test is invalid. Repeat it with a new kit.
  • A negative result does not always rule out infection. Testing too soon, or collecting too little sample, can affect it.
  • A positive or reactive result should always be followed up with a healthcare professional.
  • Still have symptoms or worried about a recent exposure? Speak to a healthcare professional, even if your result is negative.
  • Not sure what your result shows? Do not guess. Repeat the test with a new kit or speak to a healthcare professional.
  • Keep test kits away from children and pets, and dispose of used items safely.
  • CareFree Kits provides testing products and general information. Our tests do not replace professional medical advice or diagnosis.